FastQCFastQC Report
Tue 9 Aug 2022
T003F_S102_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT003F_S102_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4837946
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA205790.42536646750501145No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG139240.2878080904582234No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT135760.28061495518966106No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA133630.2762122603270066No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT129430.2675308901752934No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG118880.2457241151513473No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG117020.24187950836987432No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC104120.2152153000467554No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG103860.21467788189450648No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC96290.19903074569249019No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG83600.17280060587695686No Hit
GACTGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATC82230.16996882561318377No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA79110.16351980778619687No Hit
TACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACTA71300.14737659328979696No Hit
TTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAAGG69810.14429677387883205No Hit
GTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAGTCA65700.13580143308751275No Hit
GCCGTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAG65620.13563607365605154No Hit
ACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCT62700.12960045440771764No Hit
ATCGCCGTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCAT62270.1287116474636137No Hit
AAGCGTGATTGGGACGGGCACGGGCTGGGCGAGGCTGGACTTCTTTCGGG61330.12676867414394455No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT57570.11899678086526803No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA53300.11017072121102633No Hit
ATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGA53030.10961263312984476No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG52420.1083517674649531No Hit
CGGGCGTTATGGCGGAGGCTGGAATGGACGACGAAAATGGTATTGAAACG50580.10454850054134544No Hit
TTACGACGTTGGGTATTCTCCTAAAACAGTGGTTGTGAAGACAGCATCAT49740.10281222651100282No Hit
ACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTA49740.10281222651100282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)