FastQCFastQC Report
Tue 9 Aug 2022
T002G_S113_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT002G_S113_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3853845
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG75270.19531143572198673No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA65020.16871462137164314No Hit
TGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACT64370.16702799412015792No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA57010.14793018401103314No Hit
GTGAAAATAAGTCTTTCACTATTATATAAATCTAACTCGTATTTCCTAAC56530.14668467465609022No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG55930.14512778796241158No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT55000.14271461358720966No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG54200.1406387646623048No Hit
ATCAGTGAAAATAAGTCTTTCACTATTATATAAATCTAACTCGTATTTCC53960.14001600998483332No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC50850.13194614728926565No Hit
TTTTGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTC50840.13192019917770434No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG48270.12525153450644747No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG47800.12403197326306585No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG47780.12398007703994322No Hit
AGTGAAAATAAGTCTTTCACTATTATATAAATCTAACTCGTATTTCCTAA47230.12255293090407114No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT44460.11536530400158802No Hit
AGTTTTTGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCT42880.11126550237490092No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT41220.1069581158557233No Hit
AAAATAAGTCTTTCACTATTATATAAATCTAACTCGTATTTCCTAACTTT40630.10542717727360597No Hit
TCAGTGAAAATAAGTCTTTCACTATTATATAAATCTAACTCGTATTTCCT39380.10218366332844211No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)