FastQCFastQC Report
Tue 9 Aug 2022
T002FX_S220_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT002FX_S220_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000223
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG185600.4639741334420606No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG152380.3809287632214504No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG143030.3575550663050535No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA81780.20443860254790794No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG72530.181314891694788No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC63600.1589911362441544No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT59540.1488417020751093No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG58120.14529189997657632No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA55630.1390672470009797No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG55070.13766732504662865No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA54980.1374423375896794No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT54350.13586742539103444No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA52600.13149266928368744No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT49780.12444306229927682No Hit
TACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACTATTATATAA49680.12419307623599984No Hit
TGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACT47730.1193183480020989No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC43070.10766899745339198No Hit
GTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAGTCA42150.10536912567124382No Hit
TTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACTATTATA42090.10521913403327764No Hit
ACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCT41920.10479415772570679No Hit
ATCAGTGAAAATAAGTCTTTCACTATTATATAAATCTAACTCGTATTTCC41880.104694163300396No Hit
GCCGTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAG41420.10354422740932194No Hit
GTGAAAATAAGTCTTTCACTATTATATAAATCTAACTCGTATTTCCTAAC40280.10069438628796444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)