FastQCFastQC Report
Tue 9 Aug 2022
T001G_S112_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT001G_S112_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5002494
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG115700.23128463522395032No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA91420.18274884487617576No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG81530.16297870622133678No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA80460.16083977312116715No Hit
TGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACT80160.1602400722519607No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT76190.15230403074946217No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG75920.15176429996717639No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG73060.14604715168074164No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC67550.13503264571631668No Hit
TTTTGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTC65460.1308547296608452No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG65340.13061484931316258No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG62470.12487771099775433No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT56580.11310358393233455No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT56150.11224401268647198No Hit
TACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACTATTATATAA55050.11004510949938172No Hit
ATCAGTGAAAATAAGTCTTTCACTATTATATAAATCTAACTCGTATTTCC54460.10886569778994237No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA52930.10580722335698954No Hit
AGTTTTTGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCT52210.10436794127089409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)