FastQCFastQC Report
Tue 9 Aug 2022
T001FX_S219_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT001FX_S219_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4585343
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGCCAGAGCAGCAGCATCAGCAGCAGCTAGAGCAACCGTAGCAGGAC158560.3457974681501471No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG108360.23631819909655616No Hit
AGCAGGAGCAGCCAGAGCAGCAGCATCAGCAGCAGCTAGAGCAACCGTAG102090.22264419477452396No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG91470.19948344104246946No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG88840.19374777415778927No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA85920.18737965731244097No Hit
AGGAGCAGCCAGAGCAGCAGCATCAGCAGCAGCTAGAGCAACCGTAGCAG83950.18308335930376418No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA58210.1269479731396321No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT55650.12136496659028562No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT52270.1139936532556016No Hit
TTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAAGG50170.10941384319559083No Hit
TGCCGGCTATCACAAACCAGGATATTAAGCTAATTGACAATAATAGACAT48590.10596808134091605No Hit
ACATGGAAGTGGAACCGCAGCAGCAGCAGCCAGCGCAGCCGCACAAGCAC46090.10051592650756988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)