FastQCFastQC Report
Mon 5 Sep 2022
X48_S154_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameX48_S154_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4625445
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAAATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC64280.13897041257651968No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC62910.13600853539497282No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG56420.12197745298019975No Hit
TAGTGTGTTAAAAATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC54550.11793459872509564No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG54040.11683200211006725No Hit
GTGTGTTAAAAATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG50270.1086814349754456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)