FastQCFastQC Report
Mon 5 Sep 2022
X48_S154_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameX48_S154_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330965
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAAATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC33050.14178677071513301No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC32230.13826891437666375No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG28750.12333947528169664No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG28210.12102283818075346No Hit
TAGTGTGTTAAAAATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC27090.1162179612306491No Hit
GTGTGTTAAAAATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG25430.10909644717960157No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA23540.10098821732630048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)