FastQCFastQC Report
Mon 5 Sep 2022
X44_S172_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameX44_S172_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005729
Sequences flagged as poor quality0
Sequence length20-86
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC91260.9074014968246913No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC85800.8531125183821884No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA75950.7551736103860981No Hit
TATTTTTTCTGCATATTAGTTATGTGCTCATATTAAAAAAGCCAATTGCC65750.6537546396693344No Hit
CTGTTTAACCGGCAGTGACTCTGGGATTAGCAGTGACTATAGCGAGGCTG61330.6098064190254036No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT55340.5502476313201667No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG52470.521711117010646No Hit
TGACTTCGGAATCAGCTTAGTGAGATTGGAGAGAAAATGCATCGGCAGTA44300.44047651007378724No Hit
AAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACTG40360.4013009468753511No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA39730.3950368339781392No Hit
GCCCCAGTTGTTGCTGCACCAAAAAGTGGTGCATATGCACCAGTTGTTGC36270.36063392822519785No Hit
TATTGTAGTTCATGTGATGCTGAAGATGAACCTTGTAGCCATAGAATTTG28360.2819845107379821No Hit
AAAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACT27600.2744278031159487No Hit
AACGGTCTAGGGATGTTGATCTTCAAGTGAGAAGACCGCCATTTAAGGGG27290.27134546184906666No Hit
CTTGAGAACAAAAAATAAAAAGCAAATCAAATTATGGGTTGGTTTCACCA24780.24638844062366702No Hit
GATGGTGTATATGGCAGATGTTTTTTTATGTTAACAGGCCTTCATGGTTT24680.24539413698918897No Hit
ATAGCATCCTCTGCTGACCGGTAAATACTTGAGGAGTTTTGTGATTTCAA20810.20691458633488743No Hit
GCCCCAGTTGTTGCTGCACCTAAAAGTGGTGCATATGCACCAGTTGTTGC19800.19687211962665885No Hit
GGACTACGAAGAGGTCGGAGTCGACTCCGTCGAGGGTGAGGGAGAGGAGG15040.1495432666255025No Hit
ATATTCTTCTTGAAATCCAGACGCGTACCAGACAAATGCGTCACATGCCT14370.1428814322744994No Hit
TTAATTTAGGACATTATTTAGGATATTATAAATTAGGAATTTTTTGGCAC12140.12070846122563832No Hit
ACAGCTACGCTTGGCAACTTTGCAAAAGCAACATACGCTGCCATTGCCAA11650.11583637341669574No Hit
TTTCATTTTTAATTTTTAAACTCCGATCCCGACATTTATAATACGGCCTA11310.1124557410594703No Hit
TAAACAGAAAAGATTACCAACAAGATTCAGTAGCCCAAGTGTACTTGCAA11270.11205801960567907No Hit
AATACCAGCATATCGAGCCCGACGTGTGTAACGATATGATTATGTGAACA11240.11175972851533564No Hit
GATGACGTCAATTATAAGGTTTCCCAAGGAATTTCTTCCAGTAGACTTAG11220.11156086778844002No Hit
TTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGT11200.11136200706154441No Hit
AGTATTATCAGCAATTCCTATTAGAGTTTCGTTAGGATTTATTAGCCTTG10890.10827966579466239No Hit
CACAGTCATTATTATAATTCTGAAAATAAATGTACACATGCATAAAAATC10090.10032523671883778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)