FastQCFastQC Report
Mon 5 Sep 2022
X44_S172_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameX44_S172_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508262
Sequences flagged as poor quality0
Sequence length20-86
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC45830.9017003041738316No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC43250.8509390825991319No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA37310.7340702236248233No Hit
CTGTTTAACCGGCAGTGACTCTGGGATTAGCAGTGACTATAGCGAGGCTG32120.6319575337129276No Hit
TATTTTTTCTGCATATTAGTTATGTGCTCATATTAAAAAAGCCAATTGCC31980.6292030488212772No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT28190.554635207825885No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG26420.5198106488385911No Hit
TGACTTCGGAATCAGCTTAGTGAGATTGGAGAGAAAATGCATCGGCAGTA21940.43166713230577936No Hit
AAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACTG20380.4009743006559609No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA19760.38877586756436644No Hit
GCCCCAGTTGTTGCTGCACCAAAAAGTGGTGCATATGCACCAGTTGTTGC18210.35827978483538014No Hit
TATTGTAGTTCATGTGATGCTGAAGATGAACCTTGTAGCCATAGAATTTG14500.28528593520664536No Hit
AAAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACT14270.28076071002750547No Hit
AACGGTCTAGGGATGTTGATCTTCAAGTGAGAAGACCGCCATTTAAGGGG13650.268562276935911No Hit
GATGGTGTATATGGCAGATGTTTTTTTATGTTAACAGGCCTTCATGGTTT12610.24810038916936542No Hit
CTTGAGAACAAAAAATAAAAAGCAAATCAAATTATGGGTTGGTTTCACCA12180.23964018557358213No Hit
ATAGCATCCTCTGCTGACCGGTAAATACTTGAGGAGTTTTGTGATTTCAA10660.20973434960709242No Hit
GCCCCAGTTGTTGCTGCACCTAAAAGTGGTGCATATGCACCAGTTGTTGC10110.19891315896132308No Hit
GGACTACGAAGAGGTCGGAGTCGACTCCGTCGAGGGTGAGGGAGAGGAGG7670.1509064222782738No Hit
ATATTCTTCTTGAAATCCAGACGCGTACCAGACAAATGCGTCACATGCCT6930.1363470021366933No Hit
TTAATTTAGGACATTATTTAGGATATTATAAATTAGGAATTTTTTGGCAC6130.12060708847011974No Hit
GATGACGTCAATTATAAGGTTTCCCAAGGAATTTCTTCCAGTAGACTTAG6080.1196233438659589No Hit
AGTATTATCAGCAATTCCTATTAGAGTTTCGTTAGGATTTATTAGCCTTG5750.11313062947849731No Hit
ACAGCTACGCTTGGCAACTTTGCAAAAGCAACATACGCTGCCATTGCCAA5720.11254038271600081No Hit
TTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGT5560.1093923999826861No Hit
TTTCATTTTTAATTTTTAAACTCCGATCCCGACATTTATAATACGGCCTA5560.1093923999826861No Hit
AATACCAGCATATCGAGCCCGACGTGTGTAACGATATGATTATGTGAACA5470.10762165969519658No Hit
TAAACAGAAAAGATTACCAACAAGATTCAGTAGCCCAAGTGTACTTGCAA5390.10604766832853921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)