FastQCFastQC Report
Mon 5 Sep 2022
X44_S172_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameX44_S172_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497467
Sequences flagged as poor quality0
Sequence length20-86
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC45430.9132264049675657No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC42550.8553331175736281No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA38640.7767349392019973No Hit
TATTTTTTCTGCATATTAGTTATGTGCTCATATTAAAAAAGCCAATTGCC33770.6788389983657207No Hit
CTGTTTAACCGGCAGTGACTCTGGGATTAGCAGTGACTATAGCGAGGCTG29210.5871746266586528No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT27150.5457648447032668No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG26050.5236528252125267No Hit
TGACTTCGGAATCAGCTTAGTGAGATTGGAGAGAAAATGCATCGGCAGTA22360.449477050739044No Hit
AAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACTG19980.4016346812954427No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA19970.40143366293643595No Hit
GCCCCAGTTGTTGCTGCACCAAAAAGTGGTGCATATGCACCAGTTGTTGC18060.3630391563661509No Hit
TATTGTAGTTCATGTGATGCTGAAGATGAACCTTGTAGCCATAGAATTTG13860.2786114455833251No Hit
AACGGTCTAGGGATGTTGATCTTCAAGTGAGAAGACCGCCATTTAAGGGG13640.2741890416851771No Hit
AAAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACT13330.2679574725559685No Hit
CTTGAGAACAAAAAATAAAAAGCAAATCAAATTATGGGTTGGTTTCACCA12600.2532831323484774No Hit
GATGGTGTATATGGCAGATGTTTTTTTATGTTAACAGGCCTTCATGGTTT12070.2426291593211208No Hit
ATAGCATCCTCTGCTGACCGGTAAATACTTGAGGAGTTTTGTGATTTCAA10150.20403363439182898No Hit
GCCCCAGTTGTTGCTGCACCTAAAAGTGGTGCATATGCACCAGTTGTTGC9690.19478678987751952No Hit
ATATTCTTCTTGAAATCCAGACGCGTACCAGACAAATGCGTCACATGCCT7440.14955765910100569No Hit
GGACTACGAAGAGGTCGGAGTCGACTCCGTCGAGGGTGAGGGAGAGGAGG7370.1481505305879586No Hit
TTAATTTAGGACATTATTTAGGATATTATAAATTAGGAATTTTTTGGCAC6010.12081203376304359No Hit
ACAGCTACGCTTGGCAACTTTGCAAAAGCAACATACGCTGCCATTGCCAA5930.11920388689098975No Hit
TAAACAGAAAAGATTACCAACAAGATTCAGTAGCCCAAGTGTACTTGCAA5880.11819879509595611No Hit
AATACCAGCATATCGAGCCCGACGTGTGTAACGATATGATTATGTGAACA5770.1159875931468821No Hit
TTTCATTTTTAATTTTTAAACTCCGATCCCGACATTTATAATACGGCCTA5750.11558555642886864No Hit
TTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGT5640.11337435447979463No Hit
GATGACGTCAATTATAAGGTTTCCCAAGGAATTTCTTCCAGTAGACTTAG5140.10332343652945825No Hit
AGTATTATCAGCAATTCCTATTAGAGTTTCGTTAGGATTTATTAGCCTTG5140.10332343652945825No Hit
CACAGTCATTATTATAATTCTGAAAATAAATGTACACATGCATAAAAATC5050.10151427129839768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)