FastQCFastQC Report
Mon 5 Sep 2022
X42_S182_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameX42_S182_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631291
Sequences flagged as poor quality0
Sequence length20-86
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC200210.5513466147439023No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC189170.5209442041411718No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA161750.4454338691115639No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG129160.35568617331962654No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT123470.34001681495644387No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA92910.2558594174909144No Hit
AAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACTG88370.2433569768988495No Hit
CTGTTTAACCGGCAGTGACTCTGGGATTAGCAGTGACTATAGCGAGGCTG40580.11175088969735557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)