FastQCFastQC Report
Mon 5 Sep 2022
T60_S149_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT60_S149_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4976386
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG101110.2031795765039127No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC95490.1918862403358582No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC89020.178884837309646No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT87310.17544860868911696No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA72160.14500482880548252No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA69690.14004138746471836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)