FastQCFastQC Report
Mon 5 Sep 2022
T58_S152_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT58_S152_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736833
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG208670.5584140366989908No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC200030.5352928536008968No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC181030.4844476592879585No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT176710.4728870677389116No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA155130.415137631250848No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA143930.38516572723480014No Hit
GTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGAGAAATT44190.11825521772046008No Hit
AAGGTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGAGAA41470.11097632674513418No Hit
TTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGT40790.10915660400130271No Hit
ATGTTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTA37960.10158334611153348No Hit
TAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGTTAT37440.10019179342507412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)