FastQCFastQC Report
Mon 5 Sep 2022
T57_S157_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT57_S157_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6499974
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG108660.16716989944882857No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC98050.15084675723318278No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT85660.1317851425251855No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG83690.12875436117129083No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA81670.12564665643277959No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC76880.11827739618650783No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG75830.1166620051095589No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACC70140.10790812394018806No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC69740.10729273686325513No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA69440.10683119655555545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)