FastQCFastQC Report
Mon 5 Sep 2022
T57_S157_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT57_S157_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3229184
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG53880.16685329792294276No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC48920.15149338037101634No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT41610.12885608252735056No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG41490.12848447161883622No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA40150.12433481647375931No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC37900.11736711193911525No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG36690.11362003527826225No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC36220.11216455921991438No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACC35090.1086652231647376No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA34440.1066523307436182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)