FastQCFastQC Report
Mon 5 Sep 2022
T55_S158_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT55_S158_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5146952
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG71440.13880059499292008No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC70960.13786800420909306No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC64170.12467573041287348No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG61820.12010992136705374No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC60580.11770072850883398No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT57550.11181374918592596No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG56180.10915197965708637No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT53100.10316785546086306No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA52270.10155525056382884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)