FastQCFastQC Report
Mon 5 Sep 2022
R78_S180_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR78_S180_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610479
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG39070.14966601914820996No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC36510.1398593897901496No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC32470.12438330283446065No Hit
GGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTCTTG31630.1211655025763471No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA31520.12074412397111793No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT30060.11515128066534916No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG29730.11388714484966168No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG29080.11139718036421667No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA27370.10484665841019981No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC27040.10358252259451235No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACC26940.10319945113521313No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT26540.1016671652980162No Hit
GAACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGT26350.10093932952534765No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA26180.10028810804453894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)