FastQCFastQC Report
Mon 5 Sep 2022
R61_S207_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR61_S207_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4028136
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG60990.15140998218530854No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC57320.14229906835320358No Hit
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC55920.13882351539272755No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT50280.12482200203766707No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG50180.12457374825477592No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC49720.12343178085347664No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA49520.12293527328769435No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG45200.11221070986679695No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT42670.10592988915965101No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG42290.10498652478466468No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA41320.10257846309062058No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA40370.10022005215315472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)