FastQCFastQC Report
Mon 5 Sep 2022
R60_S201_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR60_S201_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4868039
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC118960.2443694473277638No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG107420.22066380322754192No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC106640.21906151532475396No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG105380.2164732040971734No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA91200.18734443171059229No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT86620.17793612581986298No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT85950.17655980159567333No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG80520.16540541273395715No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA73590.15116970098226412No Hit
GCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGT59370.1219587599852836No Hit
TAACAGGCTAATCCTTTAGTAGAGTTCGTATTGGCTAAAGGGATTGGCAC54420.11179039444836No Hit
AGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTT54240.11142063570156277No Hit
CAGGCTAATCCTTTAGTAGAGTTCGTATTGGCTAAAGGGATTGGCACCTC53610.11012648008777251No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC51530.10585371234700461No Hit
GCCGTCCTTTTTGGTGACTCTGGATAACTTTGAGCAGATCGCACGGGCTT48850.10034841545024599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)