FastQCFastQC Report
Mon 5 Sep 2022
R60_S201_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR60_S201_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2456158
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC60060.24452824288991182No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG55200.22474124221650238No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC53610.21826771730483135No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG52670.21444060194824602No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA45220.18410867704764922No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT44610.18162512346518425No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT43610.17755372414966789No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG40720.16578738012782565No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA36470.14848393303688118No Hit
GCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGT29710.12096127366399068No Hit
AGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTT27230.11086420336151014No Hit
TAACAGGCTAATCCTTTAGTAGAGTTCGTATTGGCTAAAGGGATTGGCAC27140.11049777742311365No Hit
CAGGCTAATCCTTTAGTAGAGTTCGTATTGGCTAAAGGGATTGGCACCTC27040.11009063749156203No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC25380.1033321146278049No Hit
GCCGTCCTTTTTGGTGACTCTGGATAACTTTGAGCAGATCGCACGGGCTT25210.10263997674416711No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)