FastQCFastQC Report
Mon 5 Sep 2022
R60_S201_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR60_S201_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411881
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC58900.244207736617188No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC53030.21986988578623903No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG52710.21854312049392155No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG52220.2165115111400604No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA45980.19063958793986935No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT42340.17554763273975788No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT42010.17417940603205548No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG39800.1650164332319878No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA37120.15390477390882884No Hit
GCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGT29660.12297455803167735No Hit
TAACAGGCTAATCCTTTAGTAGAGTTCGTATTGGCTAAAGGGATTGGCAC27280.11310674117006603No Hit
AGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTT27010.11198728295467313No Hit
CAGGCTAATCCTTTAGTAGAGTTCGTATTGGCTAAAGGGATTGGCACCTC26570.11016298067773658No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC26150.10842160123156988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)