FastQCFastQC Report
Mon 5 Sep 2022
R58_S198_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR58_S198_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5232658
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG103570.1979300003936814No Hit
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC102650.19617181172551312No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC91830.1754939841281429No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT85180.16278533777670928No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG82370.15741521804023884No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA80920.1546441598132345No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC79500.15193043382540958No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAA71180.13603029282632267No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT67100.1282331082979243No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG62850.12011104108084265No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA58660.11210363834211981No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG58230.1112818762472151No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA55070.10524288038698498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)