FastQCFastQC Report
Mon 5 Sep 2022
R58_S198_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR58_S198_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641125
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG51510.195030526764163No Hit
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC51030.1932131194093426No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC45890.1737517156514743No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT43520.1647782668370486No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG41690.15784940129679587No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA40980.1551611529177907No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC40680.15402527332102797No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAA36530.1383122722324767No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT34100.12911164749869847No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG32020.12123621562781012No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA30150.11415589947465568No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG30090.11392872355530315No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA28400.10752993516020634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)