FastQCFastQC Report
Mon 5 Sep 2022
R54_S206_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR54_S206_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5638511
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG128560.22800345694102575No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC128340.22761328301035502No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC113910.20202142019409025No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT103630.18378965652456827No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC102700.18214028490855122No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA96080.1703995966310964No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA87740.15560845762294337No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG76180.1351065910840646No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG68160.12088297779325073No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA63140.11197991810249196No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA60190.10674804039577114No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT56940.10098410732904484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)