FastQCFastQC Report
Mon 5 Sep 2022
R53_S199_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR53_S199_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4900822
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGGAGG230340.4700027872875203No Hit
CCCGTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGG180140.3675709911520965No Hit
GGCCCCGTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCAT157960.32231327724206266No Hit
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC104430.21308670259805396No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC95530.19492648376129557No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG89870.18337740077072784No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG88280.18013304706843056No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA80520.16429896862199853No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT78110.15938142621788753No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT67910.13856859114654643No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG65360.13336538237871115No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAA64180.13095762302732072No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA61640.12577481899975146No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA60620.12369353549261736No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC59630.1216734662062813No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACC55140.11251173782683803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)