FastQCFastQC Report
Mon 5 Sep 2022
R50_S174_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR50_S174_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4580898
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG189720.41415460462119No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC174290.38047125257973435No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT150310.3281234378062991No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC148790.3248053110983916No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA130060.28391813133582106No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA121750.26577758334719526No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC53400.11657103039622362No Hit
GTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGAGAAATT49990.10912707508440485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)