FastQCFastQC Report
Mon 5 Sep 2022
R50_S174_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR50_S174_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312088
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG94960.41071101099958135No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC87040.37645625945033234No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT75560.3268041700834916No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC75310.3257228963603461No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA65000.2811311680178263No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA61680.2667718529744542No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC27300.11807509056748705No Hit
GTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGAGAAATT25430.10998716311835881No Hit
CACCTCGATGTTGAATCAGGGATAATAGCTTCAAGGCGTAGAGGCTTTGA23170.10021244866112362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)