FastQCFastQC Report
Mon 5 Sep 2022
R50_S174_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR50_S174_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268810
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG94760.41766388547300126No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC87250.3845628324980937No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT74750.32946787082214907No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC73480.32387022271587307No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA65060.28675825653095677No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA60070.26476434782991964No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC26100.11503827997937244No Hit
GTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGAGAAATT24560.10825058070089606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)