FastQCFastQC Report
Mon 5 Sep 2022
R46_S178_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR46_S178_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3197726
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGGAGG52790.16508606428443212No Hit
CCCGTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGG46090.14413367499279175No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG39300.12289983569574128No Hit
GGCCCCGTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCAT38190.11942861896235013No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC37560.11745846892447945No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC37350.11680175224518924No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG34380.10751390206665612No Hit
GGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTCTTG32750.10241652974645107No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT32080.10032129081728704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)