FastQCFastQC Report
Mon 5 Sep 2022
R43_S177_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR43_S177_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5502343
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC65340.11874941275016843No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC62770.11407867521163258No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC56800.10322875182445004No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA56530.1027380517717634No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG56290.10230187394715305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)