FastQCFastQC Report
Mon 5 Sep 2022
N59_S189_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN59_S189_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5136898
Sequences flagged as poor quality0
Sequence length20-86
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG362070.7048417157591994No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC353890.6889177087028008No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC297310.5787734153958284No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT296390.5769824512770159No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA265080.5160312702335145No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA240100.4674027010074952No Hit
GTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGAGAAATT75550.14707319475683575No Hit
AAGGTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGAGAA65010.12655497539565708No Hit
CGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAAT64870.12628243737757688No Hit
CTAAAGGTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGA58670.11421289657688356No Hit
TTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGT56200.10940454725789767No Hit
TAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGTTAT53070.10331137585367668No Hit
ATGTTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTA51810.10085853369095513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)