FastQCFastQC Report
Mon 5 Sep 2022
N59_S189_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN59_S189_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587859
Sequences flagged as poor quality0
Sequence length20-86
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG180480.69741048488345No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC177270.6850064087726573No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC149120.576229230417886No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT148090.5722491063075693No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA133970.5176866282127427No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA120120.4641674836225621No Hit
GTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGAGAAATT38250.1478055798248668No Hit
AAGGTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGAGAA32510.1256250823557234No Hit
CGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAAT31860.123113353548242No Hit
CTAAAGGTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGA29240.11298915435500931No Hit
TTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGT27770.10730878305193597No Hit
TAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGTTAT26390.10197618958374471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)