FastQCFastQC Report
Mon 5 Sep 2022
N58_S195_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN58_S195_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5218598
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC105200.2015867096871612No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG101570.19463081846886846No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC100170.1919481056023093No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG99460.19058758693426855No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC95590.18317180208170855No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT85390.1636263226253488No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA85230.16331972686917062No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT83540.16008130919453847No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA81330.1558464553123272No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG78460.15034689393588085No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAA71700.13739322323735226No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG69540.13325418052894666No Hit
GGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTCTTG66070.12660488506683212No Hit
CGGGGGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCC63050.12081789016896877No Hit
GAACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGT60880.11665968522580203No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA60850.11660219852151861No Hit
AACACGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGG58880.11282723827357463No Hit
TGACGGGGGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTC57480.11014452540701544No Hit
ACGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTT54560.10454915285676344No Hit
GCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGT54010.1034952299449009No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACC53160.10186643999020428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)