FastQCFastQC Report
Mon 5 Sep 2022
N58_S195_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN58_S195_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2634024
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC53000.2012130489319763No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC51630.1960118814407158No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG50960.1934682447844059No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG50630.19221540881935775No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC47860.18169917965819596No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA43260.16423540559994898No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT43060.1634761110756774No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT42510.16138805113393045No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA40240.15277005828344767No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG40060.15208669321160323No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAA35790.13587575511840438No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG35770.13579982566597723No Hit
GGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTCTTG33410.12684015027957227No Hit
CGGGGGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCC31830.12084172353782653No Hit
GAACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGT31480.11951295812035123No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA30580.11609613276112897No Hit
AACACGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGG29700.1127552368543339No Hit
TGACGGGGGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTC29500.11199594233006228No Hit
GCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGT28190.10702256319608326No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACC27690.10512432688540424No Hit
ACGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTT27500.10440299708734621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)