FastQCFastQC Report
Mon 5 Sep 2022
N58_S195_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN58_S195_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584574
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC52200.20196751959897452No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG50940.19709244153968894No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC48540.18780657856962113No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG48500.18765181418678667No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC47730.18467259981722325No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT42880.1659074183985446No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA41970.16238652868906056No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA41090.15898171226670235No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT40480.1566215554284768No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG38400.14857380752108473No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAA35910.1389397246896394No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG33770.1306598302079956No Hit
GGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTCTTG32660.12636511858433924No Hit
CGGGGGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCC31220.12079360080229856No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA30270.11711794670998005No Hit
GAACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGT29400.1137518213833305No Hit
AACACGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGG29180.11290061727774095No Hit
TGACGGGGGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTC27980.10825768579270703No Hit
ACGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTT27060.10469810498751439No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)