FastQCFastQC Report
Mon 5 Sep 2022
N56_S192_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN56_S192_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877549
Sequences flagged as poor quality0
Sequence length20-86
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC188622.1493956462829993No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC184962.107688573515553No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA154901.7651435988189832No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG114701.307049520881455No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT114621.3061378908756092No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA79140.9018299832829847No Hit
AAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACTG62450.711641173313399No Hit
GTTTTGCTGTCCGTGACATGAGGCAGACCGTGGCTGTTGGTGTCATCAAG38800.4421405528352263No Hit
GTACATAAATGAACAGTCAATTAATGGCATTAAATGTTTTTTCTTCATAT34020.3876706599859381No Hit
CTGTTTAACCGGCAGTGACTCTGGGATTAGCAGTGACTATAGCGAGGCTG27120.3090425719817355No Hit
GCCCCAGTTGTTGCTGCACCTAAAAGTGGTGCATATGCACCAGTTGTTGC24040.2739448167566711No Hit
CGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAAT21880.2493308065988338No Hit
TTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGT19770.2252865651946501No Hit
AAAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACT18490.21070048510111686No Hit
ACAGCTACGCTTGGCAACTTTGCAAAAGCAACATACGCTGCCATTGCCAA18080.206028381321157No Hit
CACCTCGATGTTGAATCAGGGATAATAGCTTCAAGGCGTAGAGGCTTTGA16120.18369344617793423No Hit
AGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCG15950.18175623241551184No Hit
TAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGTTAT15870.18084460240966602No Hit
CTGTTTAACCGGCAGTGACTCTGGGATTAGCAGTGACTATAGTGAGGCTG15360.17503296112239886No Hit
ATGTTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTA12900.14700033844263966No Hit
TCATAATTTACTCCGCCTTTAGAGAAGTAGTGATGATGTTTTAATTGGCT11160.12717238581549292No Hit
CTAAAGGTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGA10330.11771422450484247No Hit
AAGGTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGAGAA9080.10347000566350141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)