FastQCFastQC Report
Mon 5 Sep 2022
N56_S192_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN56_S192_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444594
Sequences flagged as poor quality0
Sequence length20-86
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC95612.1505013562936073No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC92602.0827991380900324No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA77501.743163425507317No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG57991.3043360909054103No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT57711.2980382101422872No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA39780.894748916989433No Hit
AAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACTG31750.7141346936755781No Hit
GTTTTGCTGTCCGTGACATGAGGCAGACCGTGGCTGTTGGTGTCATCAAG19800.44535013967799836No Hit
GTACATAAATGAACAGTCAATTAATGGCATTAAATGTTTTTTCTTCATAT17790.40014035277129245No Hit
CTGTTTAACCGGCAGTGACTCTGGGATTAGCAGTGACTATAGCGAGGCTG13910.31286971933944224No Hit
GCCCCAGTTGTTGCTGCACCTAAAAGTGGTGCATATGCACCAGTTGTTGC12430.27958092102007676No Hit
CGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAAT11430.25708848972320814No Hit
TTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGT9590.21570241613696994No Hit
AAAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACT9150.2058057463663477No Hit
ACAGCTACGCTTGGCAACTTTGCAAAAGCAACATACGCTGCCATTGCCAA9020.2028817302977548No Hit
CTGTTTAACCGGCAGTGACTCTGGGATTAGCAGTGACTATAGTGAGGCTG8060.18128899625276096No Hit
AGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCG7980.17948960174901146No Hit
TAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGTTAT7840.17634066136744986No Hit
CACCTCGATGTTGAATCAGGGATAATAGCTTCAAGGCGTAGAGGCTTTGA7740.174091418237763No Hit
ATGTTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTA6270.14102754423136615No Hit
TCATAATTTACTCCGCCTTTAGAGAAGTAGTGATGATGTTTTAATTGGCT6000.1349545877812116No Hit
CTAAAGGTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGA5330.11988465881230966No Hit
AAGGTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGAGAA4830.10863844316387536No Hit
AGCGGCAGTTCTCCTCCGTAACGGTGGTCATTCTGTCCATAATCTCATCG4470.10054116789700265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)