FastQCFastQC Report
Mon 5 Sep 2022
N56_S192_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN56_S192_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432955
Sequences flagged as poor quality0
Sequence length20-86
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC93012.1482602118003027No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC92362.1332471042025154No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA77401.7877146585672874No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT56911.314455312907808No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG56711.3098358951854119No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA39360.909101407767551No Hit
AAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACTG30700.7090806203878001No Hit
GTTTTGCTGTCCGTGACATGAGGCAGACCGTGGCTGTTGGTGTCATCAAG19000.43884468362762874No Hit
GTACATAAATGAACAGTCAATTAATGGCATTAAATGTTTTTTCTTCATAT16230.37486574817244284No Hit
CTGTTTAACCGGCAGTGACTCTGGGATTAGCAGTGACTATAGCGAGGCTG13210.3051125405642619No Hit
GCCCCAGTTGTTGCTGCACCTAAAAGTGGTGCATATGCACCAGTTGTTGC11610.26815719878509314No Hit
CGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAAT10450.24136457599519578No Hit
TTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGT10180.23512836206996107No Hit
AAAGTCACCAAGGCTGCACAGAAAGCCGGTGGAAAGAAGTGAAAGTTACT9340.21572680763589752No Hit
ACAGCTACGCTTGGCAACTTTGCAAAAGCAACATACGCTGCCATTGCCAA9060.20925962282454294No Hit
CACCTCGATGTTGAATCAGGGATAATAGCTTCAAGGCGTAGAGGCTTTGA8380.19355360256839627No Hit
TAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGTTAT8030.1854696215542031No Hit
AGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCG7970.18408379623748428No Hit
CTGTTTAACCGGCAGTGACTCTGGGATTAGCAGTGACTATAGTGAGGCTG7300.16860874686745736No Hit
ATGTTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTA6630.15313369749743044No Hit
TCATAATTTACTCCGCCTTTAGAGAAGTAGTGATGATGTTTTAATTGGCT5160.11918097723781916No Hit
CTAAAGGTTGGATTTAAAATGCTTTCTAAGTTATTAAATTTTTAGAAAGA5000.11548544305990231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)