FastQCFastQC Report
Mon 5 Sep 2022
N54_S193_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN54_S193_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2958725
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGGAGG132980.4494503544601137No Hit
CCCGTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGG102050.34491208206237484No Hit
GGCCCCGTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCAT92810.3136824138775993No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG70660.2388190859238354No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC67200.22712485952564027No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC62150.2100566967190259No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT58060.1962331747627779No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC57630.1947798460485513No Hit
GGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTCTTG54650.1847079400755393No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG54370.18376158649418248No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG49000.165611876737446No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA48890.16524009497334158No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA46970.15875081327260898No Hit
GAACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGT46290.15645252600359955No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA44910.15178835478119798No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG44420.15013223601382353No Hit
AACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTC42480.1435753576287083No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT42210.1426628023895428No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACC41520.14033071677834202No Hit
TGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTC38780.1310699710179216No Hit
GCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGT36030.12177542691530979No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA35080.11856458440713483No Hit
AGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTT33490.11319064799871566No Hit
GTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCT33460.11308925297214172No Hit
AGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACATCCAAGG32610.1102163938858799No Hit
CGGGGGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCC31110.10514664255718256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)