FastQCFastQC Report
Mon 5 Sep 2022
N54_S193_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN54_S193_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916699
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGGAGG129270.44320651531063027No Hit
CCCGTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGG99810.34220192073299305No Hit
GGCCCCGTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCAT89600.30719659450632375No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG68790.23584881401886174No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC64920.22258038968025157No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC58690.20122062646848374No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC57510.1971749570319049No Hit
GGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTCTTG54900.1882264848035399No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT54550.18702649810624958No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG53520.18349510868279517No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG49400.1693695509889776No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA49250.16885527097585318No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA46650.15994108408169647No Hit
GAACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGT43370.14869549446137567No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA43290.1484212117877093No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT42800.14674123041150286No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG42030.14410125967746415No Hit
AACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTC41790.14327841165646507No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACC40600.13919845688567795No Hit
TGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTC37020.1269243072391083No Hit
GCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGT33870.11612442696349537No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA33420.1145815869241221No Hit
GTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCT33220.1138958802399562No Hit
AGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTT31980.1096444987981276No Hit
AGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACATCCAAGG31590.10830737076400411No Hit
CGGGGGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCC30790.1055645440273405No Hit
AACACGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGG30200.10354170930905109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)