FastQCFastQC Report
Mon 5 Sep 2022
N49_S185_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN49_S185_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250931
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC35540.15789022408949896No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG33500.14882730745633696No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC31360.1393201302039023No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT28590.12701411105004995No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC27030.12008364538939666No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA26450.11750693379761531No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG23780.1056451752630356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)