FastQCFastQC Report
Mon 5 Sep 2022
N47_S168_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN47_S168_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5118771
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG103260.20172811012643463No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC81920.1600384154712137No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC69700.13616549753837395No Hit
AGTGCCATATACCCCCACATGAACTCTTGAGTACTAGATGAATGACTATA66870.130636826691407No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG66760.13042193135813263No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA59170.11559415336220354No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG58950.11516436269565487No Hit
GCTGGCGGTCCGCCTCGTTGCGGTCACTGCCTGTCCTGACCCAACCTCTC56130.10965522778807649No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC56100.10959661996991074No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT54450.10637318997079573No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA52010.10160642075998319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)