FastQCFastQC Report
Mon 5 Sep 2022
N47_S168_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN47_S168_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537580
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG50010.19707753056061286No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC41150.16216237517634913No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC34690.13670504969301459No Hit
AGTGCCATATACCCCCACATGAACTCTTGAGTACTAGATGAATGACTATA34200.13477407608824155No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG33950.13378888547356144No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA29330.11558256291427264No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG28890.11384862743243562No Hit
GCTGGCGGTCCGCCTCGTTGCGGTCACTGCCTGTCCTGACCCAACCTCTC28120.11081424033922084No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC27600.10876504386068617No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT27540.10852859811316294No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA25710.1013170028137044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)