FastQCFastQC Report
Mon 5 Sep 2022
N46_S170_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN46_S170_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2612355
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC48450.18546483919681667No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC43460.1663633005468246No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG40720.15587468012578687No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC40150.15369274084111847No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG37340.14293616296406883No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG37190.14236196841547186No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA34760.13306001672820117No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT33550.12842818070285242No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA32580.12471505595525877No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG29700.11369052062219721No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT29490.11288664825416148No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA28270.10821653259223958No Hit
GCTGGCGGTCCGCCTCGTTGCGGTCACTGCCTGTCCTGACCCAACCTCTC26950.10316362056458636No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA26880.10289566310857445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)