FastQCFastQC Report
Mon 5 Sep 2022
N45_S166_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN45_S166_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2681377
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC51080.19049913533233112No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG48900.1823689842942637No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG48130.17949732544136837No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC47920.1787141457542151No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC47900.17863955721258146No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT40550.15122826816221666No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA40460.15089261972486523No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT39520.1473869582680839No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA37290.1390703358759324No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG36270.13526632025261648No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG34690.12937382546355847No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA33750.1258681640067771No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA30810.1149036483866312No Hit
AACACGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGG28750.1072210285983657No Hit
GCTGGCGGTCCGCCTCGTTGCGGTCACTGCCTGTCCTGACCCAACCTCTC28360.1057665520365096No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACC27290.10177606505910955No Hit
ACGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTT26910.10035888276807027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)