FastQCFastQC Report
Mon 5 Sep 2022
N43_S165_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN43_S165_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5662107
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAATAAT104580.18470156074408342No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC102000.1801449531066792No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG77010.1360094395955428No Hit
GGTCCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAG76750.1355502465778199No Hit
CCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAAT73600.1299869465554077No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG64990.11478059316081453No Hit
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC64700.11426841633335434No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG63810.11269656331114901No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA62360.11013567917384819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)