FastQCFastQC Report
Mon 5 Sep 2022
N43_S165_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN43_S165_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848219
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAATAAT52310.18365863018258077No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC51400.18046365114480312No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG38410.13485620312202118No Hit
GGTCCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAG38390.13478598380250956No Hit
CCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAAT36730.12895778028304705No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG33160.11642363175022707No Hit
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC32740.11494902604048354No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG32230.11315843339293782No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA31860.11185937598197329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)