FastQCFastQC Report
Mon 5 Sep 2022
N43_S165_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN43_S165_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2813888
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAATAAT52270.18575721563900197No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC50600.17982236677508132No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG38600.13717674619601064No Hit
GGTCCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAG38360.13632383378442925No Hit
CCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAAT36870.13102866922919462No Hit
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC31960.11357950280892488No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG31830.11311750858598495No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG31580.11222905815725431No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA30500.10839095230513794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)