FastQCFastQC Report
Mon 5 Sep 2022
M90_S147_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM90_S147_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4342231
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG80130.18453647445287918No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC70040.16129957157967875No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC65330.15045261295403214No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC64930.14953142750811738No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT63170.1454782115460923No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG59490.1370033054436763No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG56700.13057803695842068No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT50400.11606936618526283No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA49420.1138124618427716No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA49210.11332883948366634No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG48190.11097981659658365No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA45430.10462363701977163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)