FastQCFastQC Report
Mon 5 Sep 2022
M90_S147_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM90_S147_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156253
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG39520.18328090442077066No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC35390.164127307880847No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC32280.14970413954206674No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC31770.14733892544149504No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT30270.14038241338099008No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG28950.1342606827677457No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG27790.12888098010762186No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT25120.11649838863992305No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA24740.1147360722512618No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA24390.11311288610381064No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG23670.10977376031476825No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA23280.10796506717903698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)